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靈芝發酵液的成分檢測及美白與抗衰老功效評價

Analysis of fermentation liquor of Ganoderma lucidum and evaluation of its whitening and anti- senility efficacy

【作者】 趙丹許丹妮王冬冬張佳嬋李萌王昌濤

【Author】 ZHAO Dan;XU Dan-ni;WANG Dong-dong;ZHANG Jia-chan;LI Meng;WANG Chang-tao;School of Science,Beijing Technology and Business University;

【機構】 北京工商大學理學院

【摘要】 為開發靈芝發酵液在化妝品領域的應用,使用葡萄酒酵母發酵靈芝并測定發酵液中所含有的多糖、多酚、黃酮和多肽含量。通過DPPH自由基清除實驗和羥自由基清除實驗對靈芝發酵液的抗氧化能力進行檢測,并測定其對成纖維細胞存活率的影響以評估其可能在抗衰老方面發揮的作用。最后通過體外酪氨酸酶實驗和小鼠黑色素瘤細胞內的酪氨酸酶活性及黑色素合成抑制實驗對靈芝發酵液的美白功效進行評價。結果表明,靈芝發酵液富含多糖,質量濃度為9.910 g/L,多酚、黃酮和多肽的質量濃度分別為0.054,0.045和2.931 g/L。體積分數分別為25.89%和42.18%的靈芝發酵液可清除50%的DPPH自由基和羥自由基;體積分數為0.05%~0.2%范圍內的靈芝發酵液可有效促進成纖維細胞增殖;靈芝發酵液對酪氨酸酶活性抑制率高達73.22%,其通過抑制酪氨酸酶活性起到抑制黑色素合成的作用。因此靈芝發酵液具有一定的抗衰老和美白功效。

【Abstract】 To develop the application of fermentation liquor of Ganoderma lucidum in the field of cosmetics,Saccharomyces ellipsoideus were used to acquire fermentation liquor Ganoderma lucidum,and the content of total polysaccharides,total polyphenols,total flavones and total polypeptides in the fermentation liquor were determined. The scavenging activity of OH·free radicals and DPPH free radicals as well as the influence on fibroblasts viability were measured to evaluate the possible anti- senility effect of the fermentation liquor.Finally,via in vitro tyrosinase activity test and intracellular tyrosinase activity test on melanin synthesis of mouses as well as melanin inhibition test,the evaluation for whitening effect of the fermentation liquor was conducted. Results showed that the fermentation liquor of Ganoderma lucidum contains 9. 910 g / L of total polysaccharides,0. 054 g / L of total polyphenols,0. 045 g / L of total flavones,and 2. 931 g / L of total polypeptides. The fermentation liquor with 25. 89% and 42. 18% volume fraction of fermentation liquor of Ganoderma lucidum could eliminate 50% OH · free radical and DPPH free radical. The fermentation liquor with 0. 05%- 0. 2% volume fraction can promote fibroblasts proliferation effectively. The activity of tyrosinase is inhibited significantly by the fermentation liquor of Ganoderma lucidum and the inhibition rate reaches73. 22%. The fermentation liquor of Ganoderma lucidum could inhibit melanin synthesis by decreasing activity of tyrosinase. The fermentation liquor of Ganoderma lucidum exhibits efficacy for anti- senility and whitening.

【關鍵詞】 化妝品添加劑靈芝發酵抗氧化美白
【Key words】 cosmetic additiveGanoderma lucidumfermentionantioxidantwhitening
【基金】 北京工商大學2015年研究生科研能力提升計劃資助項目;中國檢驗檢疫科學研究院基本科研業務費裝箱資金資助項目(2015JK015);質檢公益性行業科研專項(201310132,201410019)
  • 【文獻出處】 日用化學工業 ,China Surfactant Detergent & Cosmetics , 編輯部郵箱 ,2016年04期
  • 【分類號】TQ658
  • 【下載頻次】707
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